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H6268

Sigma-Aldrich

Hide–Remazol Brilliant Blue R

protease substrate

Synonym(s):

Hide powder azure, Remazol Brilliant Blue R–Hide

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About This Item

CAS Number:
Beilstein:
5629635
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

Quality Level

form

powder

storage temp.

2-8°C

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Application

Hide−Remazol Brilliant Blue R has been used:
  • to study the substrate specificity of a purified collagenase preparation from Clostridium histolyticum
  • to mix with cytotoxic samples for hide powder azure protease activity assay
  • as a high molecular weight substrate to test the inhibition of trypsin by the purified recombinant rat bikunin, α1-m/bikunin precursor, and α1-m
  • as a substrate to obtain the inhibition of snake venom protease activity by the addition of exogenous citrate

Other Notes

Hide powder covalently linked to Remazol Brilliant Blue R

Substrates

Chromogenic substrate for trypsin

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Soluble, dye-labelled substrates for a micro-plate assay of proteinase activity
Wolf G A and Wirth S J
Journal of Microbiological Methods, 25(3), 337-342 (1996)
Expression of a functional proteinase inhibitor capable of accepting xylose: bikunin
Falkenberg C, et al.
Archives of Biochemistry and Biophysics, 387(1), 99-106 (2001)
Citrate inhibition of snake venom proteases
Odell G V, et al.
Toxicon, 36(12), 1801-1806 (1998)
Jacob A Galán et al.
Toxicon : official journal of the International Society on Toxinology, 43(7), 791-799 (2004-07-31)
The Southern Pacific Rattlesnake (Crotalus helleri) is found in southwestern California (USA), southward through north Baja California (MX) into the northern part of southern Baja California (MX). In this study, the venoms from two Southern Pacific Rattlesnakes were characterized. The
J Melrose et al.
Archives of orthopaedic and trauma surgery, 114(3), 145-152 (1995-01-01)
Chemonucleolysis is a therapeutic procedure whereby a degradative enzyme is injected intradiscally to reduce disc height/width by depolymerisation of extracellular matrix components. This process is considered to diminish disc pressure on inflamed nerve roots, resulting in the alleviation of sciatic

Protocols

Our General Protease Assay Procedures and Substrates overview.

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