GERPN2236
ECL™ Prime Western Blotting Detection Reagent
Cytiva RPN2236
Synonym(s):
chemiluminescent detection reagent, Western blotting detection reagent
About This Item
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packaging
pkg of 1 kit
manufacturer/tradename
Cytiva RPN2236
General description
Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.
Application
Features and Benefits
- Compatible with Rainbow Molecular Weight Markers and Amersham™ ECL DualVue™ Western Blotting Markers.
- High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
- Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
- Optimized for use with Amersham Hybond-P® (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.
- Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm™.
Components
- RPN2236 Solution A: Luminol solution, 3 × 50 ml Solution B: Peroxide solution, 3 × 50 ml sufficient for 3000 cm2 membrane
Storage and Stability
Analysis Note
Legal Information
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.
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