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GE28-9893-32

Sigma-Aldrich

HiLoad® 26/600 Superdex® 30 pg

Cytiva 28-9893-32, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

mol wt

<10000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9893-32

parameter

<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa (42 psi)

bed volume

320 mL

column L × I.D.

60 cm × 26 mm

matrix

agarose-dextran composite
 dextran-agarose particle platform
polymeric

matrix active group

dextran-agarose phase
dextran-agarose phase

avg. part. size

34 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

General description

HiLoad 26/600 Superdex 30 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography separating peptides.

Suitability

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating peptides

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Legal Information

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocols

Performing a Separation with Superdex

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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