GE28-9673-88
His Mag Sepharose™ Ni
Cytiva 28-9673-88, pack of 2 × 1 mL
About This Item
Recommended Products
ligand
IMAC immobilized with Ni
packaging
pack of 2 × 1 mL
manufacturer/tradename
Cytiva 28-9673-88
parameter
Room temperature temp. range
matrix
paramagnetic, spherical, highly cross-linked agarose particles
capacity
50 mg binding capacity(histidine-tagged protein/ml medium)
Related Categories
General description
Application
The product is based on IMAC Sepharose™ medium with magnetite incorporated and nickel immobilized.
Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel
Features and Benefits
- High capacity magnetic beads for small-scale purification and screening of histidine-tagged Proteins
- Easy to use visible and dense magnetic beads
- High purity and yield
- Easy parallel screening with high repeatability
- Scalable-simple capture of target Protein from small μL to high mL sample volumes
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
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This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from Cytiva. Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and subsequently charged with Ni2+ ions.
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