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GE28-9365-50

GSTPrep Fast Flow 16/10

Cytiva 28-9365-50

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

glutathione and 10-carbon linker arm

packaging

pkg of 20 mL

manufacturer/tradename

Cytiva 28-9365-50

parameter

<10 mL/min flow rate
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

highly cross-linked 4% agarose

average diameter

90 μm

cleaning

3-12

working range

3-12

capacity

>10 mg/mL binding capacity

General description

GSTPrep FF 16/10 is prepacked, ready-to-use column containing Glutathione Sepharose 4 Fast FLow designed for one-step, scale-up purification of glutathione S-transferase (GST)-tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

Glutathione Sepharose 4 Fast FLow is for scale-up purification of recombinant GST-tagged Proteins, other glutathione S-transferases, and glutathione-dependent Proteins. Mild elution conditions preserve Protein antigenicity and function.

Features and Benefits

  • Fast and easy one-step, scale-up purification of GST-tagged Proteins.
  • Binds approx. 200 mg recombinant GST/column
  • Mild elution conditions preserving Protein antigenicity and function.
  • Simple operation with a pump or chromatography system, such as ÄKTA® design.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

GSTPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

Protocols

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from Cytiva.

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service