G6784

L-Glutamine–Penicillin–Streptomycin solution

L-glutamine 200 mM, streptomycin 10 mg/mL, penicillin 10,000 units, 0.1 μm filtered, BioReagent, suitable for cell culture

• Synonym(s): Pen/Strep, Penicillin/Streptomycin
• MDL number: MFCD00283636
• UNSPSC Code: 12352207
• NACRES: NA.76

Properties

• biological source: synthetic
• Quality Level: 300
• description: stabilized
• sterility: 0.1 μm filtered
• product line: BioReagent
• form: liquid
• composition: L-glutamine, 200 mM ; penicillin, 10,000 units ; streptomycin, 10 mg/mL
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria
• Mode of action: cell wall synthesis | interferes; protein synthesis | interferes
• storage temp.: −20°C

Description

Application

This antibiotic solution is used to reduce or eliminate bacterial contamination in primary cell cultures. It is recommended for us at 10 mL/L.

Biochem/physiol Actions

Mode of Action: The product acts in three ways: Glutamine is an essential energy source for most mammalian cells in culture due to its amino acid carbon skeleton. Penicillin inhibits bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria.

Components

This solution is formulated to contain 200 mM L-glutamine, 10,000 units of Penicillin and 10 mg/mL of streptomycin, solubilized in a proprietary buffer.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves