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G5419

Sigma-Aldrich

Anti-GAD1/GAD2 Antibody

mouse monoclonal, K-87

Synonym(s):

Anti-GAD 67

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About This Item

MDL number:
MFCD06202147
UNSPSC Code:
12352203
NACRES:
NA.41

product name

Monoclonal Anti-Glutamic Acid Decarboxylase 67 (GAD67) antibody produced in mouse, clone K-87, purified antibody

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

K-87, monoclonal

mol wt

antigen 67 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:20,000 (with antigen-retrieval step)
western blot: 1:500-1:20,000 using mouse brain extract

isotype

IgG1κ

UniProt accession no.

Q99259

shipped in

dry ice

storage temp.

−70°C

target post-translational modification

unmodified

Gene Information

human ... GAD1(2571)
mouse ... Gad1(14415)
rat ... Gad1(24379)

General description

Glutamic Acid Decarboxylase 67 (GAD67) is encoded by the gene glutamic acid decarboxylase 1 (GAD1). GAD1 gene is located on the human chromosome at 2q31.1. GAD67 is localized in the dendrites and cell bodies.

Specificity

Reacts specifically with GAD67. Does not crossreact with GAD65.

Immunogen

This peptide does not share homology with GAD65.
synthetic peptide from mouse GAD67 (amino acids 87-106).

Application

Monoclonal Anti-Glutamic Acid Decarboxylase 67 (GAD67) antibody is suitable for use in immunoblot, western blot (at 1:500-1:20,000 dilutions using mouse brain extract) and immunohistochemistry (at 1:500-1:20,000 dilutions in mouse brain sections).
Monoclonal anti-glutamic acid decarboxylase 67 (GAD67) antibody produced in mouse has been used in:
  • immunohistofluorescence
  • western blotting
  • immunohistochemistry.

Biochem/physiol Actions

Glutamic Acid Decarboxylase 67 (GAD67) is involved in the production of γ-amino butyric acid (GABA), a inhibitory neurotransmitter in the central nervous system (CNS). Deficiency of GAD67 leads to psychosis, schizophrenia and bipolar diseases.

Physical form

Solution in phosphate buffered saline containing 0.01% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Baohu Ji et al.
Human molecular genetics, 23(21), 5683-5705 (2014-06-09)
The t(1; 11) translocation appears to be the causal genetic lesion with 70% penetrance for schizophrenia, major depression and other psychiatric disorders in a Scottish family. Molecular studies identified the disruption of the disrupted-in-schizophrenia 1 (DISC1) gene by chromosome translocation
Baohu Ji et al.
PloS one, 8(6), e66327-e66327 (2013-07-05)
It has been well established that schizophrenia patients display impaired NMDA receptor (NMDAR) functions as well as exacerbation of symptoms in response to NMDAR antagonists. Abnormal NMDAR signaling presumably contributes to cognitive deficits which substantially contribute to functional disability in
Owen Chan et al.
Diabetes, 57(5), 1363-1370 (2008-04-01)
We have previously demonstrated that modulation of gamma-aminobutyric acid (GABA) inhibitory tone in the ventromedial hypothalamus (VMH), an important glucose-sensing region in the brain, modulates the magnitude of glucagon and sympathoadrenal responses to hypoglycemia. In the current study, we examined
Mitsuhiro Tsuboi et al.
Cancer medicine, 8(9), 4189-4199 (2019-06-18)
In a previous genome-wide screening, we identified hypermethylated CpG islands around glutamate decarboxylase 1 (GAD1) in lung adenocarcinoma (LADC). In this study, we aimed to investigate the methylation and expression status of GAD1 and its prognostic value in patients with
Eric S Wohleb et al.
The Journal of clinical investigation, 126(7), 2482-2494 (2016-06-09)
Major depressive disorder (MDD) is a recurring psychiatric illness that causes substantial health and socioeconomic burdens. Clinical reports have revealed that scopolamine, a nonselective muscarinic acetylcholine receptor antagonist, produces rapid antidepressant effects in individuals with MDD. Preclinical models suggest that
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