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G4134

Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

Synonym(s):

G-6-P-DH, Zwischenferment

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About This Item

CAS Number:
9001-40-5
Enzyme Commission number:
1.1.1.49 (BRENDA, IUBMB)
EC Number:
232-602-6
MDL number:
MFCD00081656
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type IX

Quality Level

200

form

lyophilized powder

specific activity

200-400 units/mg protein (modified Warburg-Christian)

mol wt

128 kDa

β-NADP and β-NADPH content

≤10 mmol/mol

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

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General description

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.

Application

Glucose-6-phosphate dehydrogenase is used:
  • To test ketose reductase activity in developing maize endosperm.
  • For recycling microassay of β-NADP and β-NADPH.
  • To measure the intracellular levels of NADPH and total NADP.
  • To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide. Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death. Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Physical form

Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D Oh et al.
Molecular and cellular biology, 10(4), 1415-1422 (1990-04-01)
The Saccharomyces cerevisiae GAL5 (PGM2) gene was isolated and shown to encode the major isozyme of phosphoglucomutase. Northern (RNA) blot hybridization revealed that the GAL5 transcript level increased three- to fourfold in response to galactose and was severely repressed in
An improved cycling assay for nicotinamide adenine dinucleotide.
C Bernofsky et al.
Analytical biochemistry, 53(2), 452-458 (1973-06-01)
A simple ultramicro method for determination of pyridine nucleotides in tissues.
J S Nisselbaum et al.
Analytical biochemistry, 27(2), 212-217 (1969-02-01)
Pranavi Koppula et al.
Nature communications, 13(1), 2206-2206 (2022-04-24)
Targeting ferroptosis, a unique cell death modality triggered by unrestricted lipid peroxidation, in cancer therapy is hindered by our incomplete understanding of ferroptosis mechanisms under specific cancer genetic contexts. KEAP1 (kelch-like ECH associated protein 1) is frequently mutated or inactivated
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
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Protocols

Enzymatic Assay of Hexokinase

To measure hexokinase activity, a continuous spectrophotometric rate-determination assay is used at 340 nm. Hexokinase catalyzes the phosphorylation of D-hexose sugars using ATP as the phosphate source.

Enzymatic Assay of Phosphoglucomutase (EC 5.4.2.2)

This procedure may be used for all Phosphoglucomutase products except for Phosphoglucomutase, Catalog Number P4109.

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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