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Key Documents

G1910

Sigma-Aldrich

Gelzan CM

Gelrite®

Synonym(s):

Gellan Gum, Agar substitute gelling agent

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
10171502
NACRES:
NA.72

Quality Level

application(s)

agriculture

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General description

Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological assay and tissue culture media. Gellan gum is used in a wide variety of immobilization matrices. It forms a hard gel at low concentrations in the presence of an electrolyte. Gellan is a linear tetrasaccharide composed of D-glucose, L-rhamnose, and D-glucuronic acid in the ratio of 2:1:1, linked by β (1 → 4) glycosidic bond.
Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological assay and tissue culture media. Gellan gum is used in a wide variety of immobilization matrices. It forms a hard gel at low concentrations in the presence of an electrolyte. Gellan is a linear tetrasaccharide composed of D-glucose, L-rhamnose, and D-glucuronic acid in the ratio of 2:1:1, linked by β (1 → 4) glycosidic bond.

Application

Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological media. Gellan gum is used in a wide variety of immobilization matricies.
Gelzan CM has been used:
  • in the synthesis of gellan-pullulan nanogel via chemical crosslinking
  • in the synthesis of gellan-pullulan hydrogel
  • with KNOP medium to maintain and prepare Anthoceros agrestis thallus tissue sample for imaging

Gelzan CM has been used:
  • in the synthesis of gellan-pullulan nanogel via chemical crosslinking
  • in the synthesis of gellan-pullulan hydrogel
  • with KNOP medium to maintain and prepare Anthoceros agrestis thallus tissue sample for imaging

Features and Benefits

  • Transparent and colorless agar alternative
  • Mechanically robust
  • Disperses in water with ease
  • Low viscosity
  • The resulting gel has a high melting temperature

Legal Information

Gelrite is a registered trademark of CP Kelco U.S., Inc.
Gelzan is a trademark of CP Kelco U.S., Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marco A Lopez-Heredia et al.
Journal of tissue engineering and regenerative medicine, 12(8), 1825-1834 (2018-04-28)
Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.
Gustavo de Medeiros et al.
Nature communications, 6, 8881-8881 (2015-11-26)
Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light
Valeria Imperato et al.
Genes, 10(6) (2019-06-20)
Hydrocarbon-degrading bacteria are important resources for use in phytoremediation applications. Yet, for many hydrocarbonoclastic strains the genetic information regarding pollutant degradation and detoxification has not been thoroughly revealed. In this study, hydrocarbon-degrading bacteria were isolated from a long-term oil-polluted soil
Caroline Teyssier et al.
Physiologia plantarum, 141(2), 152-165 (2010-10-26)
An integrated physiological and proteomic approach was used to investigate the effects of high gellan gum concentration in the medium during maturation of somatic embryos (SE) of hybrid larch, by comparing embryos incubated in media with a high gellan gum
Timothy E L Douglas et al.
Biomedical materials (Bristol, England), 12(2), 025015-025015 (2017-02-23)
Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase. In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum

Protocols

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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