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D9143

Sigma-Aldrich

o-Dianisidine

peroxidase substrate, chromogenic, powder

Synonym(s):

3,3′-Dimethoxybenzidine, Fast Blue B

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About This Item

Empirical Formula (Hill Notation):
C14H16N2O2
CAS Number:
119-90-4
Molecular Weight:
244.29
Beilstein:
1879884
EC Number:
204-355-4
MDL number:
MFCD00008372
UNSPSC Code:
12352204
PubChem Substance ID:
24894258
NACRES:
NA.83

product name

o-Dianisidine, peroxidase substrate

form

powder

solubility

H2O: slightly soluble
aqueous acid: soluble

SMILES string

COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2/h3-8H,15-16H2,1-2H3

InChI key

JRBJSXQPQWSCCF-UHFFFAOYSA-N

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Application

o-Dianisidine has been used for neutrophil myeloperoxidase activity (MPO) assay. It has also been used for staining erythrocytes.
as a constituent in neutral benzidine staining of dried blood smears

Caution

Darkens in storage.

Warning

Probably carcinogenic.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H350

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

402.8 °F - closed cup

Flash Point(C)

206 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Trim33/Tif1-gamma is essential for macrophage and neutrophil mobilisation to developmental or inflammatory cues
Demy DL, et al.
Journal of Cell Science, jcs-203471 (2017)
RNAi-mediated silencing of TNF-alphas converting enzyme to down-regulate soluble TNF-alpha production for treatment of acute and chronic colitis
Song Y, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 239, 231-241 (2016)
Amnion epithelial cells promote lung repair via lipoxin A4
Tan JL, et al.
Stem Cells Translational Medicine, 6(4), 1085-1095 (2017)
FIP200 is required for the cell-autonomous maintenance of fetal hematopoietic stem cells
Liu Fei, et al.
Blood, 116(23), 4806-4814 (2010)
Ben Brankatschk et al.
PloS one, 6(7), e21771-e21771 (2011-07-15)
In this paper, we report that the PX domain-containing protein SNX16, a member of the sorting nexin family, is associated with late endosome membranes. We find that SNX16 is selectively enriched on tubulo-cisternal elements of this membrane system, whose highly
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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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