KYSE-270
94072021
Synonym(s):
KY-270 Cells, KYSE270 Cells
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About This Item
UNSPSC Code:
41106514
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biological source
human esophagus
growth mode
Adherent
karyotype
Hypotriploid with 16% polyploidy
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human oesophageal squamous cell carcinoma
Cell Line Description
Established from the well differentiated invasive oesophageal squamous cell carcinoma resected from the middle intra-thoracic oesophagus of a 79-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC.
Culture Medium
RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS)
Subculture Routine
Split confluent cultures 1:5 every second day using trypsin/EDTA;5% CO2; 37°C. Doubling time: 24 hours. Recommended seeding density: 2.5 x 10,000 cells/cm2.
Other Notes
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Certificates of Analysis (COA)
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