MDBK Cell Line bovine

90050801

• Synonym(s): MDBK (NBL-1) Cells, Madin Darby Bovine Kidney Cells, NBL-1 Cells
• UNSPSC Code: 41106514
• eCl@ss: 32190102

Properties

• biological source: bovine kidney
• growth mode: Adherent
• karyotype: Modal no. 51; hypodiploid
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Bovine kidney

Cell Line Description

Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).

Application

MDBK cell line has been used to study the expression of Eimeria bovis-derived antigens on the host cell surface membrane in vitro. It has also been used to study the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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