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product name
AK-D, 89071903
biological source
feline lung
growth mode
Adherent
karyotype
Diploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Foetal cat lung normal
Cell Line Description
A clonal derivative of mass cultures of foetal feline lung after 14 passages in vitro. Susceptible to panleukopenia, feline herpes and feline picornaviruses.
Application
Virus studies: panleucopenia, feline herpes and feline picornaviruses
Culture Medium
Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin and 0.03% EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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