Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

CASP3C

Sigma-Aldrich

Caspase 3 Assay Kit, Colorimetric

Synonym(s):

Colorimetric Caspase-3 Activity Assay Kit

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
 sufficient for 100 standard tests (1 ml)

Quality Level

300

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).

Application

The assay can be performed (a) in 1 mL volume and measured using a spectrophotometer, or (b) in 100 µL volume in a 96-well plate using an ELISA reader.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.

Other Notes

Use 17 megohm water only

Kit Components Only

Product No.
Description
  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS
  • A2559Ac-DEVD-pNA substrate 15 mgSDS
  • p-Nitroaniline standard 1 mg

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

415.4 °F - closed cup

Flash Point(C)

213 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Sherifa S Hamed et al.
Frontiers in physiology, 7, 325-325 (2016-08-23)
The strawberry (Fragaria ananassa) has been extensively used to treat a wide range of ailments in many cultures. The present study was aimed at evaluating the hepatoprotective effect of strawberry juice on experimentally induced liver injury in rats. To this
Wafa A Al-Megrin et al.
Frontiers in physiology, 11, 64-64 (2020-03-03)
The kidney is among the metabolic organs most susceptible to injury, particularly following exposure to xenobiotics and heavy metals. We aimed to explore the potential protective impacts of coenzyme Q10 (CoQ10) on lead acetate (PbAc)-induced nephrotoxicity in rats. Four experimental
Arnau Panisello-Roselló et al.
International journal of molecular sciences, 19(9) (2018-08-23)
Institut George Lopez-1 (IGL-1) and Histidine-tryptophan-ketoglutarate (HTK) solutions are proposed as alternatives to UW (gold standard) in liver preservation. Their composition differs in terms of the presence/absence of oncotic agents such as HES or PEG, and is decisive for graft
H Schroeter et al.
The Biochemical journal, 358(Pt 3), 547-557 (2001-09-06)
Oxidative stress has been associated with neuronal loss in neurodegenerative diseases and during age-associated cognitive decline. Flavonoids have been proposed to play a useful role in protecting the central nervous system against oxidative and excitotoxic stress, although the mechanism of
Alaa Jameel A Albarakati et al.
Molecular biology reports, 47(4), 2591-2603 (2020-03-08)
Lead (Pb) is one of the most common heavy metal pollutants affecting living organisms. It induces nephrotoxicity with significant alterations in renal structure and function. Luteolin (LUT) a flavonoid present in various plant products is well known for exhibiting numerous
View All Related Papers

Articles

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service