C6144

Chlorophyll a

from Anacystis nidulans algae

• Synonym(s): Pheophytin; universal photosynthetic pigment
• Empirical Formula (Hill Notation): C₅₅H₇₂MgN₄O₅
• CAS Number: 479-61-8
• Molecular Weight: 893.49
• Beilstein: 3586237
• EC Number: 207-536-6
• MDL number: MFCD00079050
• UNSPSC Code: 12171500
• PubChem Substance ID: 24892839

Properties

• biological source: Anacystis nidulans algae
• Quality Level: 200
• form: powder
• impurities: chlorophyll b, free
• color: blue to very dark blue
• mp: 150-153 °C
• solubility: diethyl ether: 0.1 mg/10 mL
• ε (extinction coefficient): 11.1-12.1 × 10⁴ at 428-430 nm in diethyl ether; 8.3-9.4 × 10⁴ at 660-662 nm in diethyl ether
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67
• InChI: 1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1
• InChI key: ATNHDLDRLWWWCB-AENOIHSZSA-M

Description

Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).

Chlorophyll a has been used as a standard for the calibration of fluorometer.

Biochem/physiol Actions

Chlorophyll a is an essential pigment in oxygenic photosynthesis. In photosystems, it is present as an antenna pigment as well as in reaction center I and II. Chlorophyll a is a blue-green pigment which absorbs light in 400-450nm and 640-660nm range. In photosynthesis, the energy absorbed by chlorophyll transforms carbon dioxide and water into carbohydrates and oxygen. When chlorophyll absorbs light energy, an electron in chlorophyll is excited from a lower energy state to a higher energy state. In this higher energy state, the electron is more readily transferred to another molecule. This starts a chain of electron-transfer steps, which ends with an electron transferred to carbon dioxide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Sealed ampule.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves