Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

D8662

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

product name

Dulbecco′s Phosphate Buffered Saline, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
glucose: no (Dextro)

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.

Application

D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
  • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
  • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
  • to wash cells for protein isolation

Dulbecco′s Phosphate Buffered Saline has been used:
  • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
  • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
  • to wash cells for protein isolation

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
  • provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
  • serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
  • when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
  • provides a buffering system to maintain the medium within the physiological pH range

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Anna Eskilsson et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(19), 5035-5044 (2017-04-26)
Fever occurs upon binding of prostaglandin E
E Valas Teuma et al.
Journal of cataract and refractive surgery, 45(8), 1177-1182 (2019-07-06)
To compare the capsulotomy rim strength with capsular marks (CMs) to the rim strength without CMs in porcine eyes, and to demonstrate the practicality of CMs for intraoperative toric intraocular lens (IOL) alignment. LENSAR facility, Orlando, Florida, USA. Laboratory study.
Michele Russo et al.
European journal of heart failure, 21(4), 519-528 (2019-03-08)
Butyric acid (BUT), a short chain fatty acid produced daily by the gut microbiota, has proven beneficial in models of cardiovascular diseases. With advancements in cancer survival, an increasing number of patients are at risk of anticancer drug cardiotoxicity. Here
Ivan Kopljar et al.
British journal of pharmacology, 174(21), 3766-3779 (2017-01-18)
In the pharmaceutical industry risk assessments of chronic cardiac safety liabilities are mostly performed during late stages of preclinical drug development using in vivo animal models. Here, we explored the potential of human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) to
Kimberly R Long et al.
Molecular biology of the cell, 28(19), 2508-2517 (2017-07-20)
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high-capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular
View All Related Papers

Articles

Comet Assay

The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.

Protocols

Cancer Stem Cell Tumorsphere Formation Protocol

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service