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14360C

SAFC

EX-CELL® CD CHO Serum-Free Medium for CHO Cells, Chemically Defined

Animal-component free, without L-glutamine, without hypoxanthine, without thymidine, liquid, sterile-filtered, suitable for cell culture

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About This Item

MDL number:
MFCD01634638
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
L-glutamine: no
NaHCO3: 1.6 g/L
glucose: 5.6 g/L
HEPES: 7.5 mM

shipped in

ambient

storage temp.

2-8°C

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Application

EX-CELL CD CHO is a chemically defined, animal-component free, serum-free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells and expression of antibodies or protein products in suspension cell culture. CHO suspension cultures can be subcultured directly into EX-CELL CD CHO from serum-supplemented or serum-free medium with little or no adaptation. EX-CELL CD CHO is formulated without hypoxanthine, thymidine or L-glutamine, making it an appropriate medium for selection systems such as DHFR- and Glutamine Synthetase (GS System™). For applications that do not require the selective pressure of a hypoxanthine/thymidine (HT)-deficient medium, we recommend the use of EX-CELL CD CHO with hypoxanthine and thymidine (Catalog No. 14361C).

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Articles

Selection Process Development in a GS Knock-Out CHO Host Cell Line: The Effects of MSX Addition on Clones Generated in an MSX-Free Process
Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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