06966
Atto 655-Biotin
BioReagent, suitable for fluorescence, ≥90% (HPCE)
Synonym(s):
Biotin-Atto 655
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
product line
BioReagent
Quality Level
Assay
≥90% (HPCE)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm
655 nm
solubility
DMF: soluble
DMSO: soluble
acetonitrile: soluble
fluorescence
λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
λ
in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm)
suitability
suitable for fluorescence
storage temp.
−20°C
Application
Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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