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S6814

Sigma-Aldrich

SynaptoGreen C4

≥95% (HPLC), solid

Synonym(s):

FM1-43, N-(3-triethylammoniumpropyl) -4-(4-(dibutylamino)styryl) pyridinium dibromide, Pyridinium, 4-[2-[4-(dibutylamino)phenyl]ethenyl]- 1-[3-(triethylammonio)propyl]- , dibromide

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About This Item

Empirical Formula (Hill Notation):
C30H49Br2N3
CAS Number:
Molecular Weight:
611.54
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Quality Level

Assay

≥95% (HPLC)

form

solid

color

dark red

solubility

DMSO: soluble
H2O: soluble

εmax

≥50000 at 507-513 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

[Br-].[Br-].CCCCN(CCCC)c1ccc(\C=C\c2cc[n+](CCC[N+](CC)(CC)CC)cc2)cc1

InChI

1S/C30H49N3.2BrH/c1-6-11-23-32(24-12-7-2)30-18-16-28(17-19-30)14-15-29-20-25-31(26-21-29)22-13-27-33(8-3,9-4)10-5;;/h14-21,25-26H,6-13,22-24,27H2,1-5H3;2*1H/q+2;;/p-2

InChI key

VZUVCAGXYLMFEC-UHFFFAOYSA-L

General description

SynaptoGreen C4 is an oxonol dye that helps in the rapid detection of an antibiotic′s effect on bacterial viability. It is a fluorescent dye that is used in optical imaging studies to determine synaptic activity by staining synaptic vesicles at the nerve terminal or the neuromuscular junction. It is equivalent to FM1-43.

Application

SynaptoGreen C4 has been used to label cultured neurons grown on EM grids for fluorescent labeling, live light microscopy and imaging studies.

Biochem/physiol Actions

A fluorescent nerve terminal dye suitable for monitoring synaptic activity at synapses and at neuromuscular junctions.

Other Notes

Component of NTSI, also sold individually.

Legal Information

SynaptoGreen is a trademark of Biotium, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Yalda Moayedi et al.
Scientific reports, 8(1), 9975-9975 (2018-07-04)
Oral mechanoreception is implicated in fundamental functions including speech, food intake and swallowing; yet, the neuroanatomical substrates that encode mechanical stimuli are not well understood. Tactile perception is initiated by intricate mechanosensitive machinery involving dedicated cells and neurons. This signal
Yangbin Gao et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(7), 2275-2280 (2015-02-04)
Auxin binding protein 1 (ABP1) has been studied for decades. It has been suggested that ABP1 functions as an auxin receptor and has an essential role in many developmental processes. Here we present our unexpected findings that ABP1 is neither
Tam H Nguyen et al.
PloS one, 7(5), e36913-e36913 (2012-05-26)
Bulk endocytosis contributes to the maintenance of neurotransmission at the amphibian neuromuscular junction by regenerating synaptic vesicles. How nerve terminals internalize adequate portions of the presynaptic membrane when bulk endocytosis is initiated before the end of a sustained stimulation is
Christopher R Heier et al.
EMBO molecular medicine, 5(10), 1569-1585 (2013-09-10)
Absence of dystrophin makes skeletal muscle more susceptible to injury, resulting in breaches of the plasma membrane and chronic inflammation in Duchenne muscular dystrophy (DMD). Current management by glucocorticoids has unclear molecular benefits and harsh side effects. It is uncertain
Roman Pleskot et al.
Frontiers in plant science, 3, 54-54 (2012-05-29)
Phosphatidic acid (PA) is an important intermediate in membrane lipid metabolism that acts as a key component of signaling networks, regulating the spatio-temporal dynamics of the endomembrane system and the cytoskeleton. Using tobacco pollen tubes as a model, we addressed

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