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93735

Millipore

TSN Agar

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.5 g/L
casein peptone, 15 g/L
ferric citrate, 0.5 g/L
neomycin sulfate, 0.05 g/L
polymyxin B sulfate, 0.02 g/L
sodium sulfite, 1 g/L
yeast extract, 10 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
water monitoring

microbiology

suitability

selective and differential for Clostridium spp.

Application

Highly selective medium for the detection and enumeration of Clostridium perfringens in food and other material.

Preparation Note

Dissolve 40 g in 1 litre distilled water and autoclave gently at 121°C for 10 minutes. The medium should be used on the day of preparation.

Other Notes

Enumeration of Clostridium perfringens; Enumeration of sulphite reducing clostridia occurring in foods

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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D.A.A. Mossel
Journal of the Science of Food and Agriculture, 10, 662-662 (1959)
R S MARSHALL et al.
Applied microbiology, 13, 559-563 (1965-07-01)
A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of

Articles

An article regarding the Role of Clostridium perfringens and their detection, identification, and differentiation from Sigma-Aldrich.com

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