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73009

Millipore

ECC ChromoSelect Agar

NutriSelect® Plus, suitable for microbiology

Synonym(s):

E. coli Coliform Agar ChromoSelect, ECC Agar ChromoSelect

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
chromogenic mixture, 20.3 g/L
disodium hydrogen phosphate, 3.5 g/L
lactose, 2.5 g/L
monopotassium hydrogen phosphate, 1.5 g/L
neutral red, 0.03 g/L
peptone, special, 5 g/L
sodium chloride, 5 g/L
yeast extract, 3 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

6.8±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for coliforms

Application

ECC ChromoSelect Agar is a differential medium recommended for the presumptive identification of Escherichia coli and other coliforms in food and environmental samples. The chromogenic mixture containstwo chromogens as X-glucuronide and Salmon-GAL. X-glucuronide is cleaved by the enzyme beta glucuronidase produced by Escherichia coli. Salmon-GAL is cleaved by the enzyme galactosidase produced by majority of coliforns, including Escherichia coli. Color of E. coli colonies: blue/purple

Preparation Note

Suspend 55.8 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCC9544
BCBZ4528
BCBT4079
BCBM0999V
BCBL9883V

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S A Omolajaiye et al.
BioMed research international, 2020, 4250165-4250165 (2020-12-10)
Diarrhea has been reported as the leading cause of childhood mortality and morbidity globally but with disproportionate impacts in developing nations. Among bacterial etiologic agents of diarrhea, diarrheagenic Escherichia coli is the main cause of the disease among children under

Articles

Overview of Chromogenic Media

Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms

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