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71005BC

Millipore

GDS Shiga Toxin Genes (O157) Tq

BioControl, Molecular based PCR test for the detection of Shiga Toxin producing E. coli O157:H7 in food and environmental samples

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About This Item

UNSPSC Code:
41106302

Agency

AOAC® (OMA)

Quality Level

shelf life

6‑12 mo.

packaging

pkg of 100

manufacturer/tradename

Assurance GDS®
BioControl Systems 71005-100

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

compatibility

for use with GDS Rotor-Gene

storage temp.

2-8°C

suitability

Escherichia coli (O157:H7)

General description

The Assurance® GDS Shiga Toxin Genes (O157) Tq is an automated nucleic acid amplification system for the detection of Shiga Toxin producing E. coli O157:H7. The Shiga Toxin Genes (O157) assay is designed for use in conjunction with Assurance® GDS E. coli O157:H7 Tq assay to detect shiga toxin genes (stx1 and stx2) present in presumptive positive E. coli O157:H7 samples.

Application

Assurance® GDS Shiga Toxin Genes (O157) Tq is suitable for the detection of Shiga Toxin producing E. coli O157:H7 in foods, ingredients, and environmental samples.

Features and Benefits

  • Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 6 or Top 7 O groups, samples are analyzed for the presence of eae with the GDS Top 6 or 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance® GDS Shiga Toxin Genes (Top 6 or Top 7) assay
  • Confirm Assurance® GDS E. coli O157:H7 positives samples with Assurance® GDS Shiga Toxin Genes (O157) assay
  • Single-step 10-18 h enrichment in mEHEC; enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Components

  • Shiga Toxin Genes Amplification Tubes Tq
  • O157 Concentration Reagent
  • Resuspension Buffer Tq
  • Wash Solution

Compatibility

BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of Merck KGaA, Darmstadt, Germany
Assurance GDS is a registered trademark of BioControl Systems, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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