63163
Malachite Green Broth
suitable for microbiology, NutriSelect® Basic
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About This Item
Recommended Products
sterility
non-sterile
Quality Level
form
powder
shelf life
limited shelf life, expiry date on the label
composition
dipotassium hydrogen phosphate, 1.1 g/L
malachite green oxalate, 0.03 g/L
meat extract, 9 g/L
meat peptone, 15 g/L
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbiological culture: suitable
final pH
7.0±0.2 (25 °C)
application(s)
bioburden testing
environmental
food and beverages
sample preparation
water monitoring
microbiology
suitability
selective for Pseudomonas spp.
General description
Malachite Green Broth is for the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
The culture medium has been recommended by Schubert and Blum for the examination of ground-, surface-, drinking-, bathing and process-water. In addition, it is suitable for the examination of mineral and spring-water.
The culture medium has been recommended by Schubert and Blum for the examination of ground-, surface-, drinking-, bathing and process-water. In addition, it is suitable for the examination of mineral and spring-water.
Application
For the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
Malachite Green Broth has been used for the growth of Pseudomonas aeruginosa from water samples.
Preparation Note
Dissolve 25 g in 1 litre distilled water. Pour into small containers and autoclave at 121°C for 15 minutes.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Comparative hygienic surveillance of contamination with pseudomonads in a cystic fibrosis ward over a 4-year period.
Bosshammer J et al.
Journal of Hospital Infection, 31, 261-261 (1995)
Real-time PCR detection of Pseudomonas aeruginosa in clinical and municipal wastewater and genotyping of the ciprofloxacin-resistant isolates.
Schwartz T et al.
FEMS Microbiology Ecology, 57, 158-158 (2006)
[Is the malachite green broth according to Habs and Kirchner suitable for serving as an isolation medium for Pseudomonas aeruginosa from the water (author's transl)?].
R Schubert et al.
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe B: Hygiene, praventive Medizin, 158(6), 583-587 (1974-04-01)
Characterization of mucoid Pseudomonas aeruginosa strains isolated from technical water systems.
Grobe, S., J. Wingender, and H. G. Truiper
The Journal of Applied Bacteriology, 79 (1), 94-102 (1995)
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