51208

LB Broth

NutriSelect^® Plus, High salt, powder, pack of 500 g or 2.5 kg, non-sterile, nonselective for Escherichia coli

• Synonym(s): LB-Broth, high salt, Luria Bertani Broth, high salt
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: casein enzymic hydrolysate, 10 g/L ; High salt ; sodium chloride, 10 g/L ; yeast extract, 5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• packaging: pack of 500 g or 2.5 kg
• technique(s): microbiological culture: suitable
• final pH: 7.5±0.2 (25 °C)
• application(s): food and beverages; life science and biopharma; microbiology
• suitability: nonselective for Escherichia coli (Recombinant); nonselective for coliforms

Description

Application

Luria Media are used for the cultivation and maintenance of recombinant strains of E. coli for genetic and molecular studies.

Preparation Note

Suspend 25 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Dispense as desired.

Storage and Stability

• Store prepared media below 8°C, protected from direct light.
• Store dehydrated powder, in a dry place, in tightly-sealed containers at 2-25°C.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves