11363905910
Roche
Digoxigenin-11-ddUTP
solution, =85% (HPLC), pkg of 25 μL (25 nmol; 1mM)
Synonym(s):
digoxigenin
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About This Item
UNSPSC Code:
41116100
Recommended Products
Quality Level
Assay
85% (HPLC)
form
solution
mol wt
(Molecular weight: Mr = 1074.7 (DIG-11-ddUTP-Li4))
packaging
pkg of 25 μL (25 nmol; 1mM)
manufacturer/tradename
Roche
storage temp.
−20°C
General description
Digoxigenin-11-ddUTP is a 1 mM solution of the tetralithium salt. Digoxigenin-11-ddUTP is a component of probe used for end labeling. Digoxigenin-11-ddUTP is an alternative to classical radioactive labeling for electrophoretic mobility shift assay and is highly stable compared to radioactive probes.
Application
Digoxigenin-11-ddUTP has been used:
for end labeling of DNA for fragmentation reactions
- for labeling of DNA fragments in linker histone binding assay
- for end labeling of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) for electrophoretic motility shift assay and labelling of ATP citrate lyase gene for gel shift assay
for end labeling of DNA for fragmentation reactions
Quality
Typical analysis: 85% DIG-11-ddUTP (HPLC, area%)
Function tetsted by end labeling.
Formula: C43H61N4O20P3Li4
Function tetsted by end labeling.
Formula: C43H61N4O20P3Li4
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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The Journal of Biological Chemistry, 275(17), 12497-12502 (2000)
HILS1 is a spermatid-specific linker histone H1-like protein implicated in chromatin remodeling during mammalian spermiogenesis
Yan W, et al.
Proceedings of the National Academy of Sciences of the USA, 100(18), 10546-10551 (2003)
J Maupin-Furlow et al.
Journal of bacteriology, 178(2), 340-346 (1996-01-01)
The CO dehydrogenase enzyme complex from Methanosarcina thermophila contains a corrinoid/iron-sulfur enzyme composed of two subunits (delta and gamma). The cdhD and cdhE genes, which encode the delta and gamma subunits, respectively, were cloned and sequenced. The cdhD gene is
A sequence-based variation map of 8.27 million SNPs in inbred mouse strains
Frazer KA, et al.
Nature, 448(7157), 1050-1050 (2007)
Non-radioactive electrophoretic mobility shift assay using digoxigenin-ddUTP labeled probes
Kass J, et al.
Drosophila Information Service, 83, 185-188 (2000)
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