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Roche

DNA Molecular Weight Marker II, DIG-labeled

greener alternative

solution, pkg of 500 μL (10 μg/ml)

Synonym(s):

DNA marker

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About This Item

UNSPSC Code:
41105335

form

solution

Quality Level

100

packaging

pkg of 500 μL (10 μg/ml)

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

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concentration

10 μg/mL

color

colorless

solubility

water: miscible

greener alternative category

, Aligned

storage temp.

−20°C

Related Categories

General description

DNA Molecular Weight Marker II, DIG-labeled is a solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. This fragment mixture is prepared by cleavage of λDNA with Hind III. The size of the fragment ranges from 0.12 to 23.1 kbp. A photodigoxigenin has been introduced at approx. every 200th to 300th base in the DNA fragments.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DNA Molecular Weight Marker II, DIG-labeled has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection.

Features and Benefits

Contents
Ready-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.

Sequence

The mixture contains 8 fragments with the following base pair lengths: 125, 564, 2027, 2322, 4361, 6557, 9416, and 23130 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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