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Sigma-Aldrich

READYBLUE® PROTEIN GEL STAIN

Synonym(s):

protein gel stain, protein stain

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About This Item

UNSPSC Code:
41105322
NACRES:
NA.32

quality

MQ100

Quality Level

100

technique(s)

protein staining: suitable

General description

ReadyBlue® Protein Gel Stain is a rapid and sensitive colloidal Coomassie stain for polyacrylamide gels. The stain is provided as a ready-to-use solution, allowing for a fast and simplified protocol. No prior fixing step is necessary.
  • Colorimetric or IR fluorescence (800 nm) imaging
  • Reusable up to 3 times
  • No organic solvents or phosphoric acid
  • Compatible with mass spectrometry
  • Room temperature storage—it′s ready when you are

Application

Protein gel stain

Storage and Stability

Store ReadyBlue® Protein Gel Stain at room temperature. Protect From Light.

Legal Information

READYBLUE is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

ReadyBlue Protein Gel Staining Protocols

ReadyBlue™ Protein Gel Stain is a rapid and sensitive colloidal Coomassie® stain for polyacrylamide gels. The stain is provided as a ready-to-use solution, allowing for a fast and simplified protocol.

Coating Tissue Culture Plates with Vitronectin

Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. This protocol is for coating a 96-well plate and other surfaces.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

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