PTEP24H48
Millicell® 24 well hanging cell culture inserts
pore size 8.0 μm, translucent PET membrane, diam. 6.5 mm, Tissue Culture (TC)-treated surface, sterile
Synonym(s):
cell culture inserts, plate inserts
About This Item
Recommended Products
material
polystyrene housing
translucent PET membrane
Quality Level
sterility
γ-irradiated
sterile
feature
0.2x106 pores/cm2
packaging
pack of 48 ea (individually blister packaged)
parameter
37 °C max. temp.
technique(s)
cell migration: suitable
H
15.5 mm
diam.
6.5 mm
insert maximum volume
0.4 mL
insert working volume
0.2 mL
size
24 wells
surface area
0.3 cm2
color
translucent membrane
pore size
8.0 μm pore size
binding type
Tissue Culture (TC)-treated surface
General description
In plastic tissue culture plates, cells can access media only from their apical sides, but in Millicell® tissue culture inserts, cells can access media from both their apical and basolateral sides. As result, cell growth, structure, and function more closely mimic what occurs in vivo.
The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.
Application
- Angiogenesis
- Chemotaxis
- Migration / Invasion
- Feeder Layers
- Fluorescent Detection / Immunohistochemistry
- Tumor Cell Metastasis and Invasion
Legal Information
recommended
Certificates of Analysis (COA)
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Protocols
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
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