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PHCC20060

Millipore

Scepter 2.0 Handheld Automated Cell Counter

Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter Cell Counter Sensors

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

manufacturer/tradename

Scepter

technique(s)

cell counting: suitable

particle size

6-36 μm

compatibility

for use with cells sized 4.0–25.0 μm

shipped in

ambient

General description

The Scepter 2.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.

The Scepter cell counter’s screen displays:

Cell concentration
Average cell size
Average cell volume
Histogram of size or volume distribution
This system is intended for research use only and has been tested with cell types representative of those in use today.
The Scepter Cell Counter delivers rapid cell counts in a handheld easy to use format. Its on screen instructions guide you through the sampling process and in less than 40 seconds reveil data on cell concentration, average diameter, average volume and a histogram displaying population information on your culture. The device stores up to 72 counts which can be downloaded to your computer and uploaded to Excel for further manipulation.
FREE PACK OF 60μM Sensors included.

Application

Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter Cell Counter Sensors
Research Category
Cell Culture

Components

1 Scepter Cell Counter

1 Test Bead Vial

1 USB Cable

1 Downloadable Software

2 O-rings

50 Sensors (60µM)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zhiyin Xun et al.
Mechanisms of ageing and development, 133(4), 176-185 (2012-02-18)
Retinoic acid (RA) is used in differentiation therapy to treat a variety of cancers including neuroblastoma. The contributing factors for its therapeutic efficacy are poorly understood. However, mitochondria (MT) have been implicated as key effectors in RA-mediated differentiation process. Here
Reduction of infarct size by intravenous injection of uncultured adipose derived stromal cells in a rat model is dependent on the time point of application.
van Dijk, A, et al.
Stem Cell Research, 7, 219-229 (2011)
N H Foley et al.
Cell death and differentiation, 18(7), 1089-1098 (2011-01-08)
MicroRNAs function as negative regulators of posttranscriptional gene expression, having major roles in cellular differentiation. Several neuroblastoma cell lines can be induced to undergo differentiation by all-trans-retinoic acid (ATRA) and are used for modeling signaling pathways involved in this process.
Erin N Potts-Kant et al.
Free radical biology & medicine, 52(3), 705-715 (2011-12-27)
This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the Authors. Since learning of potential discrepancies between the raw data from the animal pulmonary physiology
Oncogenes induce senescence with incomplete growth arrest and suppress the DNA damage response in immortalized cells.
Sherman, Michael Y, et al.
Aging Cell, 10, 949-961 (2011)

Articles

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Using the 40 µm aperture sensor, the Scepter cell counter can accurately and precisely count a broad range of cell types, including small cells such as PBMC and red blood cells.

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Protocols

The Scepter™ cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample.

Combine the ease of automated instrumentation with the accuracy of impedance-based cell counting using the Coulter principle in an affordable, handheld format.

Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.

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