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Key Documents

MSIPS4W10

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, sterile

Synonym(s):

Hydrophobic 96-Well Plate, PVDF 96-Well Plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32039006
NACRES:
NB.22

material

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Quality Level

description

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

sterility

sterile

product line

MultiScreen®

feature

hydrophobic
lid

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

ELISPOT: suitable

filtration area

0.28 cm2

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

binding type

high binding surface

shipped in

ambient

General description

The polyvinylidene fluoride (PVDF) membrane plates are recommended, over a mixed cellulose ester format, due to slightly improved binding of capture antibody and superior performance in spot detection, particularly for fluorescent applications.

Application

MultiScreen® HTS IP Filter Plate, 0.45 μm, white, sterile has been used in enzyme-linked immunospot (ELISpot) assay using:
  • dsDNA-producing plasma cells
  • memory B cells and bone marrow localized long-lived plasma cells
  • draining lymph node and spleen cells

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

Protocols

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

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