MABT371

Anti-EB1, EB2 and EB3 Antibody, clone KT65

clone KT65, from rat

• Synonym(s): Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, EB2, End binding protein 2, EB3, End binding protein 3, Microtubule-associated protein RP/EB family member 3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: KT65, monoclonal
• species reactivity: mouse, human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_031922
• UniProt accession no.: Q61166; Q6PER3; Q8R001
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... EBF3(253738) , MAPRE2(10982) , MAPRE3(22924)

Description

General description

EB (End-binding) proteins are evolutionarily conserved and are important for extending tips of microtubules as well as regulating microtubule dynamics and communication with other intracellular substances. EB1 (End binding protein 1) encoded by the gene EB1/MAPRE1, EB2 (End binding protein 2) encoded by EB2/MAPRE2, and EB3 (End binding protein 3 also known as Microtubule-associated protein RP/EB family member 3) encoded by EB3/RP3/EBF3-S2/EBF3/MAPRE3 bind directly with end-tracking proteins (+TIPs). +TIPs influence microtubule growth, rescue, dynamicity, and suppression. EB1, EB2, and EB3 play major roles in cellular migration and adhesion, and are thought to associate with adenomatous polyposis coli tumor suppressor.

Specificity

This antibody is expected to detect EB1, EB2 and EB3.

Immunogen

GST-tagged recombinant protein corresponding to mouse EB1, EB2, and EB3.

Application

Immunocytochemistry Analysis: A representative lot detected EB1, EB2, and EB3 in NIH/3T3 cells (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099).
Immunoprecipitation Analysis: A representative lot immunoprecipitated EB1, EB2, and EB3 in HeLa cell extracts (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099).

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This Anti-EB1, EB2 & EB3 Antibody, clone KT65 is validated for use in western blotting, ICC & IP for the detection of EB1.

Quality

Evaluated by Western Blotting in MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected EB1, EB2, and EB3 in 10 µg of MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate.

Target description

31, 37, 32 kDa calculated. The calculated MW of EB1 (31 kDa), EB2 (37 kDa), and EB3 (32 kDa). The recombinants used to test the reactivity of this antibody to EB1, EB2, and EB3 have the following MW′s. EB1 (56 kDa), EB2 (62 kDa), and EB3 (58 kDa).

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing PBS.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable