MABC576
Anti-HOIL-1 Antibody, clone 2E2
clone 2E2, from mouse
Synonym(s):
RanBP-type and C3HC4-type zinc finger-containing protein 1, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, HOIL-1, Hepatitis B virus X-associated protein 4, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein 3, HO
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2E2, monoclonal
species reactivity
mouse, human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RBCK1(10616)
General description
RanBP-type and C3HC4-type zinc finger-containing protein 1 (UniProt Q9BYM8; also known as HOIL-1, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, Hepatitis B virus X-associated protein 4, RBCC protein interacting with PKC1, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein 3) is encoded by the RBCK1 gene (also known as C20orf18, HOIL1, PBMEI, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4) (Gene ID 10616) in human. The linear ubiquitin assembly complex (LUBAC) is comprised of two RING-IBR-RING (RBR)-containing E3 ligases, HOIL-1L and HOIP. LUBAC conjugates a head-to-tail-linked linear polyubiquitin chain onto lysine residues on substrate proteins. Ovarian tumor deubiquitinating enzyme (DUB) with linear linkage specificity (OTULIN/Gumby/FAM105B) and cylindromatosis (CYLD) are two DUBs that catalyze the cleavage linear polyubiquitin chains. OTULIN and CYLD interact with the N-terminal PNGase/UBA or UBX (PUB) domain of HOIP and negatively regulate LUBAC-mediated cellular processes. LUBAC is reported to suppress IFN-dependent antiviral activity by mediating TRIM25 degradation and competing against TRIM25 for RIG-I interaction.
Specificity
Clone 2E2 reacts with isoform 1 (HOIL-1L) and isoform 2, but not isoform 3.
Immunogen
Epitope: Near C-terminus
GST-tagged recombinant protein corresponding to human HOIL-1 near the C-terminus.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-HOIL-1 Antibody, clone 2E2 is validated for use in Western Blotting, Immunocytochemistry for the detection of HOIL-1 .
Western Blotting Analysis: A representative lot detected HOIL-1 in HeLa, SH-SY5Y, and U20S cells (Kirisako, T., et al (2006). EMBO J. 25(20):48877-4887).
Western Blotting Analysis: A representative lot detected HOIL-1 in mouse liver extracts (Tokunaga, F., et al (2009). Nat. Cell Biol. 11(2):123-132).
Western Blotting Analysis: A representative lot detected HOIL-1 in MEFs (Takiuchi, T., et al (2014). Genes Cells. 19(3):254-272).
Immunocytochemistry Analysis: A representative lot detected HOIL-1 in HeLa cells with or without Sendai virus (SeV) infection (Inn, K.S., et al. (2011). Mol Cell. 41(3):354-365).
Western Blotting Analysis: A representative lot detected HOIL-1 in mouse liver extracts (Tokunaga, F., et al (2009). Nat. Cell Biol. 11(2):123-132).
Western Blotting Analysis: A representative lot detected HOIL-1 in MEFs (Takiuchi, T., et al (2014). Genes Cells. 19(3):254-272).
Immunocytochemistry Analysis: A representative lot detected HOIL-1 in HeLa cells with or without Sendai virus (SeV) infection (Inn, K.S., et al. (2011). Mol Cell. 41(3):354-365).
Quality
Evaluated by Western Blotting in THP-1 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected HOIL-1 in 10 µg of THP-1 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected HOIL-1 in 10 µg of THP-1 cell lysate.
Target description
~57 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A ubiquitin ligase complex assembles linear polyubiquitin chains.
Kirisako, T; Kamei, K; Murata, S; Kato, M; Fukumoto, H; Kanie, M; Sano, S; Tokunaga et al.
The Embo Journal null
Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation.
Tokunaga, F; Sakata, S; Saeki, Y; Satomi, Y; Kirisako, T; Kamei, K; Nakagawa, T; Kato et al.
Nature Cell Biology null
Pathogen blocks host death receptor signalling by arginine GlcNAcylation of death domains.
Li, S; Zhang, L; Yao, Q; Li, L; Dong, N; Rong, J; Gao, W; Ding, X; Sun, L; Chen, X; Chen, S; Shao, F
Nature null
Hirotsugu Oda et al.
Frontiers in immunology, 10, 479-479 (2019-04-03)
Background: HOIP is the catalytic subunit of the linear ubiquitination chain assembly complex (LUBAC) that is essential for NF-κB signaling and thus proper innate and adaptive immunity. To date only one patient with HOIP deficiency has been reported with clinical
Kyung-Soo Inn et al.
Molecular cell, 41(3), 354-365 (2011-02-05)
Upon detection of viral RNA, retinoic acid-inducible gene I (RIG-I) undergoes TRIM25-mediated K63-linked ubiquitination, leading to type I interferon (IFN) production. In this study, we demonstrate that the linear ubiquitin assembly complex (LUBAC), comprised of two RING-IBR-RING (RBR)-containing E3 ligases
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