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Key Documents

MAB16989

Sigma-Aldrich

Anti-Hepatitis B Virus Antibody, core Antigen (ayw), clone 10E11, a.a. 2-10

ascites fluid, clone 10E11, Chemicon®

Synonym(s):

HBcAg

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

300

antibody form

ascites fluid

antibody product type

primary antibodies

clone

10E11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

shipped in

wet ice

Specificity

Reacts with HBcAg (epitope recognized - amino acid positions 2-8).

Immunogen

Epitope: Core Antigen (ayw)
Recombinant purified HBcAg from E.coli, SDS-denatured

Application

Anti-Hepatitis B Virus Antibody, core antigen (ayw), clone 10E11, a.a. 2-10 detects level of Hepatitis B Virus & has been published & validated for use in ELISA, IF, IP, WB, IC, IH.
ELISA, immunoblotting, immunofluorescent and immunoperoxidase staining of infected liver tissues and transfected cultured cells. Optimal working dilutions: for immunoblotting - 1:1000; for immunofluorescence - 1:200. Optimal working dilutions must be determined by the end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Physical form

Ascitic fluid. Liquid in DMEM, containing 0.1% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differentiation of core gene products of the hepatitis B virus in infected liver tissue using monoclonal antibodies
Naoumov, N V, et al
Journal of Medical Virology, 53, 127-138 (1997)
Izzat Fahimuddin Bin Mohamed Suffian et al.
Scientific reports, 7, 43160-43160 (2017-03-04)
An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E.
V Bichko et al.
Molecular immunology, 30(3), 221-231 (1993-02-01)
Particulate and denatured core protein as well as e-antigen (HBe) of hepatitis B virus (HBV) differ in part immunologically but this has not been studied in sufficient detail. Therefore, in this study the B-cell immune response to native and denatured
The affinities of monoclonal antibodies against core antigen of hepatitis B virus.
Apsalons, U and Bichko, V
Archives of Virology, 134, 393-402 (1994)

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