EZRGRA

Rat/Mouse Ghrelin ELISA Kit

measures and quantifies active Ghrelin in 20 μL serum or plasma

• Synonym(s): Appetite-regulating hormone
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.32

Properties

• product name: Rat/Mouse Ghrelin (active) ELISA, This Rat/Mouse Ghrelin (active) ELISA is used to measure & quantify Ghrelin levels in Metabolism & Endocrine research.
• Quality Level: 200
• species reactivity: rat, mouse
• packaging: kit of 1 × 96 wells
• parameter: 20 μL sample volume (4hr assay)
• technique(s): ELISA: suitable
• input: sample type serum; sample type plasma (K2 EDTA)
• UniProt accession no.: Q9EQX0 (mouse); Q9QYH7 (rat); Q9UBU3
• application(s): research use
• assay range: inter-assay cv: 1-10%; intra-assay cv: 0.3-7%; (Serum); inter-assay cv: 1-5%; intra-assay cv: 1-6%; (Plasma)
• detection method: colorimetric (450nm/590nm)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.

Millipore is pleased to offer a new Rat/Mouse Ghrelin (Active) ELISA kit for the quantification of active ghrelin in rat and mouse serum or plasma samples. Each kit is sufficient to measure 39 unknown samples in duplicate.

Specificity

Rat/Mouse Ghrelin (Active) - 100%
Rat/Mouse "Des-Octanoyl" Ghrelin - 0%
Canine Ghrelin (Active) - 111%
Porcine Ghrelin (Active) - 98%

Application

Used to detect/quantify: Ghrelin

Other Notes

Please contact Technical Service for linearity of dilution.

Safety Information

• Pictograms: GHS05,GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H290 - H302 + H332 - H311 - H315 - H317 - H319 - H335 - H411
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3