ABT143

Anti-L1CAM Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1, CD171
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, rat
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_076493
• UniProt accession no.: P32004
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... L1CAM(3897)

Description

General description

Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules (N-CAM), myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts.

Specificity

This antibody recognizes the extracellular domain of L1CAM in addition antibody recognizes the full length form (~220 kDa) and the cleaved form (~140 kDa) of L1CAM.

Immunogen

Epitope: Extracellular domain

GST-tagged recombinant protein corresponding to the extracellular domain of human L1CAM.

Application

Anti-L1CAM Antibody is a highly specific rabbit polyclonal antibody, that targets Neural cell adhesion molecule L1 & has been tested in western blotting & IHC (Paraffin).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western Blotting Analysis: 0.4 µg/mL from a representative lot detected L1CAM in 10 µg of rat brain tissue lysate.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected L1CAM in human kidney and human cerebellum tissue.

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.4 µg/mL of this antibody detected L1CAM in 10 µg of human brain tissue lysate.

Target description

~220 / 140 kDa observed. The full length protein and the cleaved form of L1CAM have been observed at ~220 kDa and ~140 kDa, respectively (Kalus, I., et al. (2003). The Journal of Biological Chemistry. 278(12):10381-10388). Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable