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AB153-I

Sigma-Aldrich

Anti-Fluorescent Gold Antibody

from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity (predicted by homology)

all

technique(s)

immunohistochemistry: suitable (paraffin)

shipped in

wet ice

target post-translational modification

unmodified

General description

Fluorescent Gold (hydroxystilbamidine), better known as Fluorogold, has been a very popular fluorescent marker used to retrogradely label projection neurons. AB153 also reacts with aminostilbamidine quite nicely in frozen, 4% PFA fixed tissues.

Specificity

This antibody will react with any species sample treated with Fluorescent Gold.

Immunogen

KLH-conjugated Fluorescent Gold.

Application

Anti-Fluorescent Gold Antibody is a highly specific rabbit polyclonal antibody, that targets Fluoro-Gold & has been tested in IHC (Paraffin).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Fluorescent Gold treated neurons in rat frontal cortex tissues (Chang, H. T., et al. (1990). J Neurosci Methods. 35(1):31-37.).

Quality

Evaluated by Immunohistochemistry in fixed frozen Fluorescent Gold treated rat cerebral cortex tissues.

Immunohistochemistry Analysis: A 1:10,000 dilution of this antibody detected Fluorescent Gold in fixed frozen Fluorescent Gold treated rat cerebral cortex tissues.

Physical form

Format: Purified

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Corticothalamic pathways, responsible for the top-down control of the thalamus, have a canonical organization such that every cortical region sends output from both layer 6 (L6) and layer 5 (L5) to the thalamus. Here we demonstrate a qualitative, region-specific difference
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