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AB139

Sigma-Aldrich

Anti-Glycine Antibody

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

shipped in

dry ice

target post-translational modification

unmodified

Specificity

Glycine

The cross-reactivities were determined using either ELISA or RIA techniques, at concentration/unconjugated or conjugated amino acid concentration at half displacement.



Compound Cross-reactivity

Glycine-G-BSA 1

Beta Alanine-G-BSA 1/100

GABA-G-BSA 1/500

Taurine-G-BSA 1/1000

Aspartate-G-BSA 1/20000

Glutamate-G-BSA <1/20000

Glycine <1/50000



The antisera was also tested for specificity using the free-floating PAP technique on rat spinal cord.



Abbreviations:

(G) Glutaraldehyde

(BSA) Bovine Serum Albumin

Immunogen

Glycine-gluteraldehyde-BSA

Application

Immunohistochemistry: 1:500-1:2,500 by PAP (see suggested protocol).

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-Glycine Antibody is validated for use in IH for the detection of Glycine.

Physical form

Rabbit antiserum. Liquid with 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ying Zhang, Masashi Kawasaki
Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology null
S McDavid et al.
Neuroscience, 139(3), 1049-1059 (2006-03-15)
Three series of experiments were carried out to characterize interneurons located within the trigeminal motor nucleus of young rats aged 5-24 days. Cholera toxin injections were made bilaterally into the masseter and, sometimes, digastric muscles to label motoneurons. In the
Atsuo Nishino et al.
BMC neuroscience, 11, 6-6 (2010-01-21)
Rhythmic motor patterns for locomotion in vertebrates are generated in spinal cord neural networks known as spinal Central Pattern Generators (CPGs). A key element in pattern generation is the role of glycinergic synaptic transmission by interneurons that cross the cord
Heterogeneous kinetics and pharmacology of synaptic inhibition in the chick auditory brainstem.
SP Kuo, LA Bradley, LO Trussell
The Journal of Neuroscience null
J H Lue et al.
Synapse (New York, N.Y.), 41(2), 139-149 (2001-06-16)
This study was aimed to clarify whether the primary afferent terminals (PATs), GABAergic terminals, and glutamatergic terminals made direct synaptic contacts with glycine-IR neurons in the cuneate nucleus of rats. In this connection, injection of the anterograde tracer WGA-HRP into

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