71058

RosettaBlue Competent Cells - Novagen

Escherichia coli, rod shaped

• UNSPSC Code: 41106202
• NACRES: NA.81

Properties

• product name: RosettaBlue Competent Cells - Novagen, RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
• biological source: Escherichia coli
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: transformation efficiency: >1×10⁶ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Genotype: endA1 hsdR17 (r_K12^– m_K12⁺) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA⁺B⁺ lacI^qZΔM15::Tn10] pRARE (Cam^R, Tet^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and recA endAlacI^q mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, and GGA on a chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.

RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosettaBlue Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany