528215

Anti-PAI-1 Mouse mAb (MA-33B8)

liquid, ≥90% (SDS-PAGE), clone MA-33B8

• Synonym(s): Anti-Plasminogen Activator Inihibitor-1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MA-33B8, monoclonal
• Assay: ≥90% (SDS-PAGE)
• form: liquid
• does not contain: preservative
• species reactivity: rabbit, human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −70°C
• target post-translational modification: unmodified
• Gene Information: human ... SERPINE1(5054)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~50 kDa PAI-1 protein.

Recognizes the ~50 kDa PAI-1 protein.

This Anti-PAI-1 Mouse mAb (MA-33B8) is validated for use in Immunoblotting, Neutralization Studies for the detection of PAI-1.

Immunogen

Human

human tPA-PAI-1 complex

Application

Immunoblotting (10 µg/ml)

Neutralization Studies (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM NaCl, 50 mM sodium phosphate buffer, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Montes, R., et al. 2000. Thromb. Haemost.84, 65.
Verhamme, I., et al. 1999. J. Biol. Chem.274, 17511.
Debrock, S., and Declerck, P.J. 1997. Biochim. Biophys. Acta1337, 257.

Rapidly inactivates PAI-1 (Plasminogen Activator Inhibitor-1) to the latent form. Like the native latent form, PAI-1 inactivated by antibody was protected from cleavage by elastase. Also shown to reduce renal fibrin deposits in a rabbit model of endotoxemia. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable