444287

MMP-13, Human, Recombinant, Active

• UNSPSC Code: 12352202

Properties

• form: liquid
• Quality Level: 100
• specific activity: ≥50 mU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Recombinant, human MMP-13. MMP-13 is a collagenase-3 enzyme that degrades a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X, and XIV, gelatin, aggrecan, perlecan and fibronectin. It is distinguished from other human collagenases by the fact that it effectively degrades type II collagen. The importance of MMP-13 is further strengthened by reports that it is a biomarker for poor prognosis in many carcinomas and other type of cancers.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1µmol MCA-Pro-Leu-Lys-Gly-Leu-DPA-Ala-Arg-NH₂ (Cat. No. 03-32-5032) at 37°C, pH 7.5.

Physical form

In 50 mM HEPES, 10 mM CaCl₂, 20% glycerol, 0.005% BRIJ^® 35 Detergent, pH 7.5. APMA-free.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Freije, M.P.S., et al. 1994. J. Biol. Chem.269,16766.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable