324740

Elastase Substrate V, Fluorogenic

Elastase Substrate V, Fluorogenic, CAS 72252-90-5, is a sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM).

• Synonym(s): Elastase Substrate V, Fluorogenic, MeOSuc-AAPV-AMC
• Empirical Formula (Hill Notation): C₃₁H₄₁N₅O₉
• CAS Number: 72252-90-5
• Molecular Weight: 627.69
• MDL number: MFCD00077135
• UNSPSC Code: 12352200

Properties

• Quality Level: 200
• Assay: ≥95% (HPLC); ≥95% (TLC)
• form: lyophilized solid; powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: white to off-white
• solubility: ethanol: 1 mg/mL; DMF: 5 mg/mL; DMSO: 5 mg/mL
• fluorescence: λ_ex 360-380 nm; λ_em 430-460 nm
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C31H41N5O9/c1-16(2)27(34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)30(42)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27,34H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,35,41,42)/t18-,19-,22-,27-/m0/s1
• InChI key: NHMQNKPYBPDCAY-NFHWZJRKSA-N

Description

General description

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K_m = 140 µM; K_cat/K_m = 120,000 s^-1 M^-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM; k_cat/K_m = 120,000 M^-1 s^-1). Not hydrolysed by cathepsin G.

Biochem/physiol Actions

Cell permeable: no

Km = 140 µM; kcat/Km = 120,000 M⁻¹ s⁻¹as substrates for human leukocyte and porcine pancreatic elastase

Primary Target
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

MeOSuc-Ala-Ala-Pro-Val-AMC

Reconstitution

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

Other Notes

Barrett, A.J. 1981. Methods Enzymol. 80, 581.
Castillo, M.J., et al. 1979. Anal. Biochem.99, 53.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable