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Millipore

M FC agar

GranuCult® plus, suitable for microbiology, for coliforms

Synonym(s):

Membrane Fecal Coliform Agar, m-Fecal Coliform Agar

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

form

granular

Quality Level

200

packaging

pkg of 500 g

manufacturer/tradename

GranuCult® plus

storage condition

dry media (Store tightly closed)

technique(s)

microbiological culture: suitable

color

blue

pH

7.2-7.6 (25 °C, 52 g/L in H2O)

solubility

52 g/L

application(s)

bioburden testing
food and beverages
microbiology

storage temp.

15-25°C

suitability

coliforms

General description

m-FC Agar is a selective membrane filtration medium suitable for the detection of fecal coliforms. The fecal coliforms derived from the intestinal tract of warm-blooded animals are differentiated from the other environmental coliforms due to their ability to grow at higher temperatures. The medium is supplemented with rosolic acid and incubated at 44.5 °C ± 0.2 °C for 24 h. Peptone and yeast extract, which serve as nutritious sources, and bile salts are added to inhibit accompanying Gram-positive flora. Lactose can be fermented by fecal coliforms at elevated temperatures to form blue colonies on the prepared medium (agar base with rosolic acid), whereas other organisms form grey colonies.

Application

m-FC Agar is used for the cultivation and enumeration of fecal coliforms by the membrane filtration technique.

Features and Benefits

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): blue to violet
pH-value (25 °C): 7.2 - 7.6
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good; colony colour blue to dark blue
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good; colony colour pink to red
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): no growth
Growth (Enterobacter aerogenes ATCC 13048): good; colony colour grey to grey-blue
Incubation: 24 hrs.; 44.5 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

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