1.03871
GranuCult® prime CIN (Cefsulodin, Irgasan, Novobiocin) Agar
acc. SCHIEMANN, ISO 10273
Synonym(s):
CIN Agar, Cefsulodin, Irgasan, Novobiocin Agar, SCHIEMANN’S Agar, Yersinia Selective Agar (YSA)
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Agency
ISO 10273
Quality Level
form
powder
technique(s)
microbiological culture: suitable
pH
7.4±0.2 (25 °C)
application(s)
food and beverages
pharmaceutical
storage temp.
15-25°C
suitability
selective for (Microorganism: Yersinia)
General description
GranuCult® prime CIN (Cefsulodin, Irgasan, Novobiocin) Agar is a nutrient rich selective medium which is designed for the isolation and differentiation of Yersinia enterocolitica from the food chain and other materials.
The selectivity of the medium is contributed using antibiotics- Cefsulodin, Irgasan(Triclosan), Novobiocin, bile salts and crystal violet dye which synergistically inhibit the accompanying flora. The pyruvate in the medium promotes the growth of Yersinia spp. agar is the solidifying agent.
Mannitol from the medium is degraded by the growing Yersinia to form an acid which will trigger a pH change indicated by neutral red. The positive colonies appear red in color. The fermentation reaction by the growing Yersinia is intense and localized which produces the characteristic “bull′s eye” appearance. The microbes which do not metabolize mannitol from the media will form colorless, translucent colonies.
The selectivity of the medium is contributed using antibiotics- Cefsulodin, Irgasan(Triclosan), Novobiocin, bile salts and crystal violet dye which synergistically inhibit the accompanying flora. The pyruvate in the medium promotes the growth of Yersinia spp. agar is the solidifying agent.
Mannitol from the medium is degraded by the growing Yersinia to form an acid which will trigger a pH change indicated by neutral red. The positive colonies appear red in color. The fermentation reaction by the growing Yersinia is intense and localized which produces the characteristic “bull′s eye” appearance. The microbes which do not metabolize mannitol from the media will form colorless, translucent colonies.
Application
GranuCult® prime CIN (Cefsulodin, Irgasan, Novobiocin) Agar is for the isolation and differentiation of Yersinia enterocolitica from the food chain and other materials.
Analysis Note
- The antibiotics- Cefsulodin, Irgasan(Triclosan), Novobiocin are added separately after autoclaving.
- The dehydrated medium is granulate with pink color.
- The prepared medium is clear to slightly opalescent and red.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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