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Key Documents

07-452

Sigma-Aldrich

Anti-dimethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

Synonym(s):

H3K27me2, Histone H3 (di methyl K27)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
cell based assay: suitable
dot blot: suitable
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

dimethylation (Lys27)

Gene Information

human ... H3C1(8350)

General description

Anti-dimethyl-Histone H3 (Lys27)

Specificity

Broad species cross-reactivity expected, based on sequence homology
Recognizes dimethylated histone H3 (Lys27).

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...ARme2KSA... in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3

Application

Additional Referenced Research Applications:
Dot Blot, Immunocytochemistry (cells)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).

Quality

Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.

Target description

17 kDa

Physical form

Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.
Peptide affinity purified

Storage and Stability

Stable for 2 years at 2-8°C from date of shipment.

Analysis Note

Control
HeLa whole cell lysate, U2OS cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes.
Silva, Jose, et al.
Developmental Cell, 4, 481-495 (2003)
Epigenetic regulation of dendritic cell-derived interleukin-12 facilitates immunosuppression after a severe innate immune response.
Wen, H; Dou, Y; Hogaboam, CM; Kunkel, SL
Blood null
Reciprocal patterns of methylation of H3K36 and H3K27 on proximal vs. distal IgVH genes are modulated by IL-7 and Pax5.
Xu, CR; Schaffer, L; Head, SR; Feeney, AJ
Proceedings of the National Academy of Sciences of the USA null
Mathieu Lupien et al.
Cell, 132(6), 958-970 (2008-03-25)
Complex organisms require tissue-specific transcriptional programs, yet little is known about how these are established. The transcription factor FoxA1 is thought to contribute to gene regulation through its ability to act as a pioneer factor binding to nucleosomal DNA. Through
Pedro Crevillén et al.
Nature, 515(7528), 587-590 (2014-09-16)
The reprogramming of epigenetic states in gametes and embryos is essential for correct development in plants and mammals. In plants, the germ line arises from somatic tissues of the flower, necessitating the erasure of chromatin modifications that have accumulated at
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