Skip to Content
Merck
All Photos(1)

Documents

341738

Sigma-Aldrich

Sulforhodamine B, acid form

laser grade, Dye content 95 %

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C27H30N2O7S2
CAS Number:
2609-88-3
Molecular Weight:
558.67
EC Number:
220-025-2
MDL number:
MFCD00067690
UNSPSC Code:
12352103
PubChem Substance ID:
57649121
NACRES:
NA.23

grade

laser grade

form

solid

composition

Dye content, 95%

mp

>300 °C (lit.)

λmax

558 nm

SMILES string

CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(O)(=O)=O)=[N+](/CC)CC)c1

InChI

1S/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35)

InChI key

IOOMXAQUNPWDLL-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Sulforhodamine B (SRB) is a fluorescent protein dye. It was reported that the dye binds itself to the amino acid residues of tricholoroacetic acid fixed cells in mild basic conditions and extracts itself into the solution under mild acidic conditions.

Application

pH sensitive of SRB towards it reactivity to living cells renders it useful for scaling drug induced cytotoxicity and cell proliferations. SRB assay was used to monitor the enzymatic activity of living cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Wieland Voigt
Methods in molecular medicine, 110, 39-48 (2005-05-20)
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH
Efficacy of 5-FU or Oxaliplatin Monotherapy over Combination Therapy in Colorectal Cancer.
Toloudi M, et al.
Journal of Cancer Therapy, 6(4), 345-345 (2015)
Minerva Nájera-Martínez et al.
Inhalation toxicology, 24(11), 762-773 (2012-09-08)
Halomethanes (HMs) can be formed during the chlorination process to obtain drinking water. In liver cells, HMs had been shown to be mutagenic and carcinogenic; however, their bioactivation by CYP 2E1 and GSTT1 is required. Although inhalation is the most
E E Gurdal et al.
Drug research, 63(3), 121-128 (2013-03-01)
This study presents the synthesis of nineteen 1-(substitutedbenzoyl)-4-benzhydrylpiperazine and 1-[(substitutedphenyl)sulfonyl]-4-benzhydrylpiperazine derivatives. In vitro cytotoxic activities of the compounds were screened against hepatocellular (HUH-7), breast (MCF-7) and colorectal (HCT-116) cancer cell lines by sulphorhodamine B assay. Among the test compounds, benzamide
Fujun Han et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 81(6), 532-540 (2012-05-11)
Multiparametric image analysis is highly preferable in revealing complicated biological processes. The multiplexing capability is demanding and is limited by spectral overlap of fluorescent dyes and the number of fluorescent channels available in an imaging platform, especially when both nuclear
View All Related Papers

Articles

Fluorescence Quenching Microscopy: Imaging Two-Dimensional Materials

Developed in the last several years, fluorescence quenching microscopy (FQM) has enabled rapid, inexpensive, and high-fidelity visualization of two-dimensional (2D) materials such as graphene-based sheets and MoS2.

Graphene in Biotechnology

Graphene has emerged as the new wonder material. Being only one atom thick and composed of carbon atoms arranged in a hexagonal honeycomb lattice structure, the interest in this material has exploded exponentially since 2004 when it was first isolated and identified using a very simple method.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service