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Merck

Transfer Membranes & Kits

PVDF and nitrocellulose Immobilon® transfer membranes for Western blotting applications.

Immobilon® PVDF and nitrocellulose transfer membranes provide high sensitivity and low background in Western blotting and other blotting applications. Tailored for specific applications, detection chemistries, and blotting techniques, Immobilon® membranes are offered in a variety of options for optimal results, flexibility, and convenience.  

We offer four types of Immobilon® PVDF membranes as well as Immobilon® nitrocellulose membranes, each optimized for a specific protein blotting application. Blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper are also available. 

  • Immobilon® NC membrane is made of nitrocellulose, exhibiting lowest background due to decreased non-specific antibody binding with the ability to bind both proteins and nucleic acids for a variety of blotting applications.
  • Immobilon®-E membrane (0.45 µm) is the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. This membrane is well-suited for most Western blotting applications.
  • Immobilon®-P membrane (0.45 µm) is optimized for Western blotting and offer better handling and staining than nitrocellulose. All Immobilon®-P PVDF membranes have a pore size of 0.45 μm.
  • Immobilon®-PSQ membrane (0.2 µm) is ideal for protein sequencing and immunoblotting of low molecular weight proteins. It has a higher protein binding capacity and a higher retention than 0.45 µm membranes.
  • Immobilon®-FL membrane (0.45 µm) was developed for fluorescence-based immunodetection. It has low background fluorescence across a wide range of excitation and emission wavelengths.
  • Immobilon® NOW rolls (8.5cm x 10m) provide improved convenience over standard transfer membrane rolls. Get a standard mini or mid blot with a single cut using measurement marks on the package lid. 

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Immobilon® Family of Transfer Membranes

Membrane Name

Immobilon® -NC

Immobilon® -E

Immobilon® -P

Immobilon® -FL

Immobilon® -PSQ

Chemistry

Nitrocellulose and MCE

PVDF

PVDF

PVDF

PVDF

Pore Size

0.45 µm

0.45 µm

0.45 µm

0.45 µm

0.2 µm

Detection Methods

Chemiluminescence, Colorimetric

Chemiluminescence, Colorimetric

Chemiluminescence, Colorimetric

Chemiluminescence, Colorimetric, Fluorescence (including near-infrared)

Chemiluminescence, Colorimetric

Binding Capacity

Goat IgG

237 µg/ cm2

225-293 μg/cm2

294 μg/cm2

300 μg/cm2

448 μg/cm2

BSA

N/A

N/A

215 μg/cm2

205 μg/cm2

340 μg/cm2

Insulin

N/A

N/A

160 μg/cm2

155 μg/cm2

262 μg/cm2

Applications

Western, Southern and Northern blots, dot blots

Western blots

Western blots, dot blots

Western blots, dot blots

Western blots, dot blots

The durability and high protein retention of Immobilon® PVDF membranes make them the preferred option for sequential detection of multiple targets via antibody stripping and reprobing.

Selection Criteria

•High-abundance target protein

•Wide range of molecular weights

•Target protein expressed over a broad dynamic range

•Wide range of molecular weights

•Target protein expressed over a broad dynamic range

•Wide range of molecular weights

•Target protein expressed over a broad dynamic range

•Wide range of molecular weights

•Target protein <20 kDa

•Very low abundance target protein

•Low affinity antibody

Immobilon® Nitrocellulose Membranes for Western Blotting

Nitrocellulose (NC) membranes bind proteins through hydrophobic interactions. Immobilon® nitrocellulose membranes:

  • bind both proteins and nucleic acids 
  • are compatible with Western blotting, Northern blotting, Southern blotting, and dot blot techniques 
  • exhibit low background attributed to decreased non-specific antibody binding
  • demonstrate excellent binding capacity for low molecular weight proteins
  • are optimized for chemiluminescent, chromogenic, and fluorescent detection



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