Transfer Membranes & Kits

Immobilon® PVDF and nitrocellulose transfer membranes provide high sensitivity and low background in Western blotting and other blotting applications. Tailored for specific applications, detection chemistries, and blotting techniques, Immobilon® membranes are offered in a variety of options for optimal results, flexibility, and convenience.
We offer four types of Immobilon® PVDF membranes as well as Immobilon® nitrocellulose membranes, each optimized for a specific protein blotting application. Blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper are also available.
- Immobilon® NC membrane is made of nitrocellulose, exhibiting lowest background due to decreased non-specific antibody binding with the ability to bind both proteins and nucleic acids for a variety of blotting applications.
- Immobilon®-E membrane (0.45 µm) is the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. This membrane is well-suited for most Western blotting applications.
- Immobilon®-P membrane (0.45 µm) is optimized for Western blotting and offer better handling and staining than nitrocellulose. All Immobilon®-P PVDF membranes have a pore size of 0.45 μm.
- Immobilon®-PSQ membrane (0.2 µm) is ideal for protein sequencing and immunoblotting of low molecular weight proteins. It has a higher protein binding capacity and a higher retention than 0.45 µm membranes.
- Immobilon®-FL membrane (0.45 µm) was developed for fluorescence-based immunodetection. It has low background fluorescence across a wide range of excitation and emission wavelengths.
- Immobilon® NOW rolls (8.5cm x 10m) provide improved convenience over standard transfer membrane rolls. Get a standard mini or mid blot with a single cut using measurement marks on the package lid.
Products
Immobilon® Family of Transfer Membranes | ||||||
---|---|---|---|---|---|---|
Membrane Name | Immobilon® -NC | Immobilon® -E | Immobilon® -P | Immobilon® -FL | Immobilon® -PSQ | |
Chemistry | Nitrocellulose and MCE | PVDF | PVDF | PVDF | PVDF | |
Pore Size | 0.45 µm | 0.45 µm | 0.45 µm | 0.45 µm | 0.2 µm | |
Detection Methods | Chemiluminescence, Colorimetric | Chemiluminescence, Colorimetric | Chemiluminescence, Colorimetric | Chemiluminescence, Colorimetric, Fluorescence (including near-infrared) | Chemiluminescence, Colorimetric | |
Binding Capacity | Goat IgG | 237 µg/ cm2 | 225-293 μg/cm2 | 294 μg/cm2 | 300 μg/cm2 | 448 μg/cm2 |
BSA | N/A | N/A | 215 μg/cm2 | 205 μg/cm2 | 340 μg/cm2 | |
Insulin | N/A | N/A | 160 μg/cm2 | 155 μg/cm2 | 262 μg/cm2 | |
Applications | Western, Southern and Northern blots, dot blots | Western blots | Western blots, dot blots | Western blots, dot blots | Western blots, dot blots | |
The durability and high protein retention of Immobilon® PVDF membranes make them the preferred option for sequential detection of multiple targets via antibody stripping and reprobing. | ||||||
Selection Criteria | •High-abundance target protein •Wide range of molecular weights | •Target protein expressed over a broad dynamic range •Wide range of molecular weights | •Target protein expressed over a broad dynamic range •Wide range of molecular weights | •Target protein expressed over a broad dynamic range •Wide range of molecular weights | •Target protein <20 kDa •Very low abundance target protein •Low affinity antibody |
Immobilon® Nitrocellulose Membranes for Western Blotting
Nitrocellulose (NC) membranes bind proteins through hydrophobic interactions. Immobilon® nitrocellulose membranes:
- bind both proteins and nucleic acids
- are compatible with Western blotting, Northern blotting, Southern blotting, and dot blot techniques
- exhibit low background attributed to decreased non-specific antibody binding
- demonstrate excellent binding capacity for low molecular weight proteins
- are optimized for chemiluminescent, chromogenic, and fluorescent detection
To continue reading please sign in or create an account.
Don't Have An Account?