G006153

LC/MS (TOF) Analysis of Opioids and Opiate-Dependence Management Drugs on Ascentis^® Express HILIC

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 μm particles (53934-U); mobile phase: 2 mM ammonium formate in 90:10 (v:v) acetonitrile:water; flow rate: 0.3 mL/min; column temp.: 55 °C; detector: TOF/MS; injection: 2 μL; sample: 400 ng/mL in acetonitrile
• suitability: application for HPLC
• application(s): clinical testing; forensics and toxicology

Description

Analysis Note

The rapid analysis by HPLC with TOF-MS detection of seven opioids is shown here using an Ascentis Express HILIC column. Cerilliant CRMs were used to ensure reliable MS identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Oxycodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
6-Acetylcodeine solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant^®
6-Acetylmorphine, 1.0 mg/mL solution, 1 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant^®
Codeine solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Hydrocodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Morphine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Hydromorphone solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®