ZRB1417
Anti-p-Rb-Ser807/811 Antibody, clone 3J7 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Retinoblastoma-associated protein;p105-Rb;p110-RB1;pRb;pp110
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About This Item
UNSPSC Code:
12352203
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biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3J7, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 106.16 kDa
observed mol wt ~110 kDa
purified by
using Protein A
species reactivity
rat, human
species reactivity (predicted by homology)
monkey
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer807/pSer811)
Gene Information
human ... RB1(5925)
Related Categories
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3J7 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Retinoblastoma-associated protein phosphorylated on serine 807 and 811.
Immunogen
KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phosphoserines 807 and 811 from human Retinoblastoma-associated protein (Rb).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cells treated with nocodazole.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Rb-Ser807/811 in HeLa cells treated with Nocodazole (40 ng/mL; 8 h).
Tested Applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected phospho-Rb-Ser807/811 in one million serum starved MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Rb-Ser807/811 in C6 cells.
Affinity Binding Assay: A representative lot of this antibody bound phospho-Rb-Ser807-811 peptide with a KD of 9.1 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-Rb-Ser807/811 in human lung cancer and human ovarian cancer tissue sections.
Peptide Inhibition Assay: Target band detection in a lysate from HeLa cells treated with Nocodazole (40 ng/mL; 8 h) was prevented by pre-blocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cells treated with nocodazole.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Rb-Ser807/811 in HeLa cells treated with Nocodazole (40 ng/mL; 8 h).
Tested Applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected phospho-Rb-Ser807/811 in one million serum starved MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Rb-Ser807/811 in C6 cells.
Affinity Binding Assay: A representative lot of this antibody bound phospho-Rb-Ser807-811 peptide with a KD of 9.1 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-Rb-Ser807/811 in human lung cancer and human ovarian cancer tissue sections.
Peptide Inhibition Assay: Target band detection in a lysate from HeLa cells treated with Nocodazole (40 ng/mL; 8 h) was prevented by pre-blocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Retinoblastoma-associated protein (UniProt: P06400; also known as p105-Rb, p110-RB1, pRb, Rb, pp110) is encoded by the RB1 gene (Gene ID: 5925) in human. Retinoblastoma (Rb) protein act as a negative regulators of cell cycle and controls the passage of cells from G1 to S phase. It is a key regulator of entry into cell division that acts as a tumor suppressor. It acts as a transcription repressor of E2F1 target genes. In its underphosphorylated, active state it interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Its action is regulated by phosphorylation of several serine and threonine residues on the protein. Phosphorylated Rb protein sequesters transcription factors allowing the transition from G1 to S phase. It is phosphorylated by Cdk6 and Cdk4, and subsequently by Cdk2 at serine 567 in G1, thereby releasing E2F1 which is then able to activate cell growth. It is dephosphorylated in the late M phase. Phosphorylation at threonine 821 and 826 promotes interaction between the C-terminal domain and the pocket domain (aa 373-771), and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Phosphorylation of serine 807 and 811 residues by Cdk3/cyclin-C is shown to be essential for G0-G1 transition. Mutations in Rb1 gene are known to cause childhood retinoblastoma, a malignant tumor that arises from the nuclear layers of the retina. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chinnam, M., and Goodrich, DW. (2011). Curr. Top. Dev. Biol. 94; 129-169; Harbour, JW., et al. (1999). Cell. 98(6); 859-869).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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