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GE17-5199-01

MabSelect

Cytiva 17-5199-01, pack of 25 mL

Synonym(s):

MabSelect Column

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-5199-01

matrix

highly cross-linked agarose

avg. part. size

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning in place

2-12

working range

3-10

capacity

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

General description

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
MabSelect is a protein A affinity medium designed for capturing MAbs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

MabSelect is a BioProcess affinity medium for capturing monoclonal antibodies (MAbs) from large sample volumes. MabSelect is developed for cost-effective high throughput processing of large amounts of feed. This is the reason why MabSelect has been designed-in to several new and second-generation processes for the production of therapeutic MAbs.

The medium is based on a highly cross-linked Agarose matrix with a recombinant Protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity medium with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool. MabSelect is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.

The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native Protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect an excellent choice for purification of MAbs at process scale.

MabSelect is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • High-fLow Agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
  • Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity which reduces media volume requirements
  • Low non-specific binding resulting in Low levels of impurities in the product eluate pool
  • BioProcess medium supported for industrial applications and well-established in approved processes.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

MabSelect is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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