GE29-0491-04

HiTrap^® MabSelect SuRe^™

Cytiva 29-0491-04, pack of 1 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: alkali-stabilized protein A-derived (E. coli)
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• packaging: pack of 1 mL
• manufacturer/tradename: Cytiva 29-0491-04
• availability: not available in North America
• storage condition: (20% Ethanol); (20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)
• parameter: <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.); 42 psi
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: highly cross-linked agarose
• avg. part. size: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-13.7(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
• working range: 3-12(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
• capacity: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
• suitability: suitable for bioprocess medium
• storage temp.: 2-8°C

Description

General description

HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP).

Features and Benefits

• Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
• Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Legal Information

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.

HiTrap is a registered trademark of Cytiva

MabSelect SuRe is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids